Fish reproduction

The CRISPR/Cas-based techniques represent a major breakthrough in targeted genome editing of living organisms. In the case of wheat, the technology has already enabled the specific reconstruction of previously characterized mutations, resulting in economic benefits. In terms of implementation, the most efficient method is to integrate the gene encoding the Cas enzyme responsible for DNA cleavage and the guideRNA's gene that designates the target sequence, into the plant genome and then to remove it after mutagenesis. However, as a result of transgenic integration, small and untraceable pieces of DNA can be incorporated into chromosomes, which are difficult to identify. Therefore, various DNA-free transient techniques have been developed, such as the biolistic delivery of the Cas-guideRNA ribonucleoprotein complex into tissue cultures, but its mutation frequency is very low. Our aim is to develop a more efficient genome editing method for wheat that does not require transformation of the target genome. To achieve this, barley is transformed with a wheat genome-specific CRISPR system and then, via sexual crossing, this barley genome is introduced into the wheat egg cell. As a result, the wheat genome can be edited throughout the life cycle of the hybrid plant. Thereafter, the wheat genome is once again introduced by backcrossing with the original parent, and after self-fertilization coupled with paternal genome elimination, edited wheat progeny without barley chromosomes is selected. Our preliminary results demonstrate that this system is capable of generating specific gene mutations in the wheat genome very efficiently.

The investigation of post-thaw chilled storage and the applicability of large-scale cryopreservation in chub (Squalius cephalus) sperm

Evaluation of the efficacy of acetone-dried common carp pituitary during induced breeding of African catfish (Clarias gariepinus) after an extremely long-term storage

Vitrification of the ovarian tissue in sturgeons

Fish genetics

Validation of a male-specific DNA marker confirms XX/XY-type sex determination in several Hungarian strains of African catfish (Clarias gariepinus)

Evaluation of triploid rainbow trout Oncorhynchus mykiss as a surrogate parent for brown trout Salmo trutta m. fario and grayling Thymallus thymallus

Genetic Structure and Diversity of Native Tench (Tinca tinca L. 1758) Populations in Hungary—Establishment of Basic Knowledge Base for a Breeding Program

Fish and water toxicology

Interaction of Fumonisin B1, N-Palmitoyl-Fumonisin B1, 5-O-Palmitoyl-Fumonisin B1, and Fumonisin B4 Mycotoxins with Human Serum Albumin and Their Toxic Impacts on Zebrafish Embryos

Microinjection based zebrafish embryo test for the detection of estrogenic substances in slurry based irrigation water and its combined application with yeast estrogen screen

Embryonic exposure to low concentrations of aflatoxin B1 triggers global transcriptomic changes, defective yolk lipid mobilization, abnormal gastrointestinal tract development and inflammation in zebrafish